Metabolomics is the study of small-molecules (metabolites) of a biological organism, with the metabolome representing the collection of all metabolites.
The Targeted Metabolomics Laboratory (TML) is located in Rightmire Hall and serves the entire Ohio State University community. The TML focuses in the detection and quantitation of selected small molecules (mass-to-charge ratio [m/z] up to 1,250) from a biological source. Our methods are designed to take advantage of a state-of-the art liquid chromatography (Agilent UHPLC 1290) coupled to a highly sensitive mass spectrometer (AB Sciex QTRAP 5500). Moreover, the laboratory has a High Pressure Liquid Chromatography-Photodiode Array Detector (Waters Alliance HPLC/PDA system) to separate and monitor metabolites that have UV-Vis absorption. The TML can detect and quantify over 180 metabolites (complete list) including sugars, sugar alcohols, amino acids, organic acids, phosphorylated compounds, polyamines, phenolics, hormones, secondary metabolites, fatty acids, and polyunsaturated aldehydes.
In addition, the staff can design and validate methods for new metabolites that clients want to pursue.
For information regarding analytical projects, sample submission, instrument training and use, please contact Jean-Christophe Cocuron.