1) Liquid Chromatography-Mass Spectrometry System (LC/MS/MS)

The AB Sciex QTRAP 5500 system is a bench top hybrid triple Quadrupole-Linear Accelerator-trap mass spectrometer that is highly sensitive with a mass range of m/z 5-1250 in triple quadrupole mode and m/z 5-1000 in linear ion trap (LIT) mode. Available ionization sources include ESI and APCI. The scan modes include: full scan MS and selected ion scan for both Q1 and Q3, Product Ion Scan, Precursor Ion Scan, Neutral Loss Scan, Multiple Reaction Monitoring and MS3 scan. This instrument is ideal for metabolites present at a very low level (fmol). An Agilent 1290 UHPLC is interfaced to support fast LC/MS/MS analysis of complex matrixes. Analyst software is used to pilot the LC/MS/MS system and analyze data.

2) High Pressure Liquid Chromatography-Photodiode Array Detector  (HPLC-PDA)

The  Waters Alliance 2695 HPLC system is a high pressure liquid chromatography platform with full wavelength detection range from 190 nm to 800 nm that provides superior separation for metabolites which have UV-Vis absorption. Empower 3 software is used to pilot the HPLC-PDA system and analyze data.